Winnie Wong, political adviser for the liberal National Women's March, said the protest would translate into "the energy we will need to see to at the ballot box in November". They were arrested by U.S. Capitol Police on charges of "crowdinig, obstructing, or incommoding".
Loretta Fudoli took a bus to Washington from Conway, Arkansas, to join Thursday's protest.
According to reports, more than 500 people were arrested by the Capitol Police when they were having their sit-in protest at the Senate Hart Office building.
The Trump administration's practice of separating families began last month after Attorney General Jeff Sessions implemented a "zero tolerance" policy under which all adults who cross the U.S. -Mexico border without passing through an official port of entry are prosecuted.
Rep. Pramila Jayapal (D-Wash.) was arrested along with about 575 others. Following Trump's signing of an executive order last week-only after the policy sparked global outrage-Immigration and Customs Enforcement (ICE) agents will no longer separate families.
With the zero tolerance policy now in tatters, the women are turning to an even bigger target, demanding the abolition of ICE and for the release of all illegal immigrants from detention.
President Trump is spending the weekend at his golf club in Bedminster, N.J., and won't be home during the protest.
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Two of those arrested included actress Susan Sarandon and Rep. Pramila Jayapal, who serves the 7th Congressional District of Washington state. "And as a member of Congress, I refuse to let this president and this administration do what they are doing to children, to parents, to asylum seekers in my name".
"When we were advocating to keep families together and end family separations, we were not advocating for family incarceration", said Linda Sarsour, one of the leaders of the Women's March.
In response to her arrest, the Congresswoman sent out a second tweet noting she was among 500 arrested.
"Their parents shouldn't even be locked up", Fudoli said.
Most of the children who had been separated from their families before the order was signed have not yet been reunited with them.
Larger protests are being planned for Saturday in Washington, D.C., and cities around the country under the banner of #FamiliesBelongTogether.